Reporter

Part:BBa_K256033:Design

Designed by: iGEM09_NTU-Singapore   Group: iGEM09_NTU-Singapore   (2009-10-20)

Infrared signal reporter (J23119:IFP:J23119:HO1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1182
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 763
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 563
    Illegal AgeI site found at 608
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is just a test construct for our system.

The test constructs is to determine the successful production of infrared fluorescent protein (IFP) or heme oxygenase-1 (HO-1) and thus the functionality of our imaging device.

As infrared fluorescent cannot be generated by producing IFP alone, a constitutively expressed HO1 generator was ligated to the IFP generator. This, as a whole, makes up a constitutively ON infrared signal reporter.

Source

Gene sequence is obtained directly from Tsien's lab.

References